steps are necessary for the HBOD test, and small (28-mL)
HBOD tubes are used that take up little space.
First, the wastewater is dispensed into HBOD the tubes.
We use a digital dispensette (shown far left), although any
standard pipettor works just fine.
The volume of wastewater put in the tube varies depending
on the strength of the wastewater (see procedure). For
for a raw wastewater (39 to 236 mg-HBOD/L) you would fill to
for low-BOD wastewater (7 to 50 mg-HBOD/L) fill with 23 mL.